期刊
PLANTA
卷 222, 期 2, 页码 301-306出版社
SPRINGER
DOI: 10.1007/s00425-005-1524-6
关键词
Arabidopsis; 5-azacytidine; ddm1 mutant; DNA methylation pattern; plant development
We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs.
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