期刊
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
卷 289, 期 4, 页码 F835-F849出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00122.2005
关键词
AE2; chloride/bicarbonate exchanger
资金
- NHLBI NIH HHS [HL-32262] Funding Source: Medline
- NIDDK NIH HHS [DK-53093, DK-43495] Funding Source: Medline
Although the zebrafish has been used increasingly for the study of pronephric kidney development, studies of renal ion transporters and channels of the zebrafish remain few. We report the cDNA cloning and characterization of the AE2 anion exchanger ortholog from zebrafish kidney, slc4a2/ae2. The ae2 gene in linkage group 2 encodes a polypeptide of 1,228 aa exhibiting 64% aa identity with mouse AE2a. The exon-intron boundaries of the zebrafish ae2 gene are nearly identical to those of the rodent and human genes. Whole-mount in situ hybridization detects ae2 mRNA in prospective midbrain as early as the five-somite stage, then later in the pronephric primordia and the forming pronephric duct, where it persists through 72 h postfertilization (hpf). Zebrafish Ae2 expressed in Xenopus laevis oocytes mediates Na+-independent, electroneutral Cl-36(-)/Cl- exchange moderately sensitive to inhibition by DIDS, is inhibited by acidic intracellular pH and by acidic extracellular pH, but activated by ( acidifying) ammonium and by hypertonicity. Zebrafish Ae2 also mediates Cl-/HCO3- exchange in X. laevis oocytes and accumulates in or near the plasma membrane in transfected HEK-293 cells. In 24 - 48 hpf zebrafish embryos, the predominant but not exclusive localization of Ae2 polypeptide is the apical membrane of pronephric duct epithelial cells. Thus Ae2 resembles its mammalian orthologs in function, mechanism, and acute regulation but differs in its preferentially apical expression in kidney. These results will inform tests of the role of Ae2 in zebrafish kidney development and function.
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