期刊
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
卷 61, 期 3, 页码 126-133出版社
ELSEVIER
DOI: 10.1016/j.ejpb.2005.06.006
关键词
lentiviral vector; Pluronic 17127; viral vector delivery; central nervous system
资金
- Medical Research Council [G9805564] Funding Source: researchfish
- MRC [G9805564] Funding Source: UKRI
- Medical Research Council [G9805564] Funding Source: Medline
Lentiviral vectors have been demonstrated as efficient tools for gene delivery to the CNS. We describe a novel approach for vector delivery using the thermoresponsive Gel, Pluronic F127 as a carrier. A HIV-1 lentiviral vector expressing GFP was contained in various Concentrations of gel (15, 30 and 40%) and applied to cultures of 293T cells. FACS analysis of cells transduced with 8 ng of lentiviral vector revealed a similar transduction efficiency for each Gel concentration compared to vector added to cells without PF127. Primary Rat CNS mixed glial cultures were also transduced with lentiviral vector in 15% Pluronic F127 and results demonstrated a similar transduction efficiency of astrocytes compared to virus without gel and no evidence of cell toxicity or death. Stereotaxic delivery of viral vector in 15% PF127 to the rat brain resulted in transduction of cells, predominantly astrocytes close to the injection site. Pluronic F127 get delivery of viral vectors to the CNS may provide a platform for localised release particularly in areas of brain or spinal cord injury. (c) 2005 Elsevier B.V. All rights reserved.
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