期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 58, 期 9, 页码 5537-5546出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.03457-14
关键词
-
资金
- Deutsche Forschungsgemeinschaft [SPP 1580, HE6008/1-1]
- Federal Ministry of Education and Research (BMBF)
- Yousef Jameel Scholarship
Chlamydia trachomatis is a medically important human pathogen causing different diseases, including trachoma, the leading cause of preventable blindness in developing countries, and sexually transmitted infections that can lead to infertility and ectopic pregnancies. There is no vaccine against C. trachomatis at present. Broad-spectrum antibiotics are used as standard therapy to treat the infection but have unwanted side effects, such as inducing persistent or recurring infections and affecting the host microbiome, necessitating the development of novel anti-Chlamydia therapies. Here, we describe the establishment of a robust, fast, and simple plaque assay using liquid overlay medium (LOM) for the identification of anti-Chlamydia compounds. Using the LOM plaque assay, we identified nitrobenzoxadiazole (NBD)-labeled 1-O-methyl-ceramide-C-16 as a compound that efficiently inhibits C. trachomatis replication without affecting the viability of the host cell. Further detailed analyses indicate that 1-O-methyl-NBD-ceramide-C-16 acts outside the inclusion. Thereby, 1-O-methyl-NBD-ceramide-C-16 represents a lead compound for the development of novel anti-Chlamydia drugs and furthermore constitutes an agent to illuminate sphingolipid trafficking pathways in Chlamydia infections.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据