期刊
JOURNAL OF CELL SCIENCE
卷 118, 期 19, 页码 4333-4341出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.02550
关键词
axonemal function; beta tubulin isotype; cilia; ciliary beat frequency
类别
资金
- NCI NIH HHS [CA26376] Funding Source: Medline
- NCRR NIH HHS [1P20RR16469, C06 RR017417, 1 C06 RR17417-01, P20 RR016469] Funding Source: Medline
- NIAAA NIH HHS [AA-08769, R01 AA008769, R37 AA008769, R29 AA008769] Funding Source: Medline
- NIDCD NIH HHS [DC02053, R01 DC002053-10, R01 DC002053, R29 DC002053] Funding Source: Medline
In previous studies in Drosophila, Nielsen et al. hypothesized that the beta tubulin C-terminal axonemal motif 'EGEFXXXI, where X is an acidic amino acid, is required for ciliary function and assembly (Nielsen et al., 2001, Curr. Biol. 11, 529-533). This motif is present in some but not all mammalian beta tubulin isotypes. We therefore investigated whether this motif is important in ciliary function in mammals. In a preparation of isolated, ATP-reactivated bovine tracheal cilia, we found that monoclonal antibodies directed against the C-terminus of beta I, beta IV and beta V tubulin blocked ciliary beating in a concentration dependent manner. Antibodies against other epitopes of beta tubulin were ineffective, as were antibodies against alpha tubulin. Peptides consisting of the axonemal motif and motif-like sequences of these isotypes blocked ciliary beating. These results suggest that the axonemal motif sequences of beta I, beta IV and beta(V) tubulin are essential for ciliary function. Peptides consisting of corresponding C-terminal sequences in a tubulin isotypes were also ineffective in blocking ciliary beating, which suggests that the C-terminus of alpha tubulin is not directly involved in cilia function in mammals.
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