期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 15, 期 5, 页码 548-555出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2005.08.009
关键词
-
资金
- NIBIB NIH HHS [R01 EB001903] Funding Source: Medline
- NIGMS NIH HHS [U54 GM074961] Funding Source: Medline
Protein crystallization is important for determining protein structures by X-ray diffraction. Nanoliter-sized plugs aqueous droplets surrounded by a fluorinated carrier fluid have been applied to the screening of protein crystallization conditions. Preformed arrays of plugs in capillary cartridges enable sparse matrix screening. Crystals grown in plugs inside a microcapillary may be analyzed by in situ X-ray diffraction. Screening using plugs, which are easily formed in PDMS microfluidic channels, is simple and economical, and minimizes consumption of the protein. This approach also has the potential to improve our understanding of the fundamentals of protein crystallization, such as the effect of mixing on the nucleation of crystals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据