期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 57, 期 7, 页码 3230-3239出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00243-13
关键词
-
资金
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R21AI105342]
- Department of the Army [W81XWH-12-2-0138]
- Princeton University
The antibiotic tolerances of bacterial persisters have been attributed to transient dormancy. While persisters have been observed to be growth inhibited prior to antibiotic exposure, we sought to determine whether such a trait was essential to the phenotype. Furthermore, we sought to provide direct experimental evidence of the persister metabolic state so as to determine whether the common assumption of metabolic inactivity was valid. Using fluorescence-activated cell sorting (FACS), a fluorescent indicator of cell division, a fluorescent measure of metabolic activity, and persistence assays, we found that bacteria that are rapidly growing prior to antibiotic exposure can give rise to persisters and that a lack of replication or low metabolic activity prior to antibiotic treatment simply increases the likelihood that a cell is a persister. Interestingly, a lack of significant growth or metabolic activity does not guarantee persistence, as the majority of even dormant subpopulations (> 99%) were not persisters. These data suggest that persistence is far more complex than dormancy and point to additional characteristics needed to define the persister phenotype.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据