期刊
FEBS LETTERS
卷 579, 期 24, 页码 5414-5418出版社
WILEY
DOI: 10.1016/j.febslet.2005.09.002
关键词
11-cis retinal; isomerase; mutagenesis; retinal dystrophy; vitamin A; visual cycle
资金
- NEI NIH HHS [EY012231, EY015650] Funding Source: Medline
We have recently reported that RPE65 from the retinal pigment epithelium is the isomerohydrolase, a critical enzyme in the visual cycle for regeneration of 11-cis retinal, the chromophore for visual pigments. Here, we demonstrated that mutation of any one of the absolutely conserved four histidine and one glutamic acid residues to alanine in RPE65 abolished its isomerohydrolase activity. Substitution of the conserved glutamic acid with glutamine also resulted in loss of the activity. Moreover, these mutations significantly reduced protein stability of RPE65. These results indicate that these conserved residues are essential for the isomerohydrolase activity of RPE65 and its stability. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据