期刊
GENE
卷 359, 期 -, 页码 91-98出版社
ELSEVIER
DOI: 10.1016/j.gene.2005.06.003
关键词
polyketide synthase; myxobacteria; anticancer; non-ribosomal peptide synthetase; polyketide
资金
- NCI NIH HHS [R43 CA105615-01] Funding Source: Medline
Disorazoles are polyketides produced by the myxobacterium Sorangium cellulosum So ce12. Their mode of action is to inhibit tubulin polymerization and destabilize microtubules. Using transposon mutagenesis, two mutant strains were identified that produced no disorazoles. Sequencing the DNA flanking the insertions revealed a polyketide synthase gene cluster that would encode three polypeptides, DszA, DszB, and DszC, with DszC containing both nonribosomal peptide synthetase and polyketide synthase modules. The disorazole polyketide synthase modules lack an acyltransferase domain. Instead, a separate gene, dszD, encodes an AT protein, thus revealing that the disorazole gene cluster falls into the trans-AT Type I family of PKS enzymes. (c) 2005 Elsevier B.V. All rights reserved.
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