期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 56, 期 11, 页码 5907-5915出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00770-12
关键词
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资金
- DFG grant [MA2873/1-3]
- UK Biotechnology and Biological Sciences Research Council grant
- Marie Curie Intra-European Fellowship
- Marie Curie Short Term Fellowship
- BBSRC [BB/G015902/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G015902/1] Funding Source: researchfish
L-forms are cell wall-deficient bacteria that can grow and proliferate in osmotically stabilizing media. Recently, a strain of the Gram-positive model bacterium Bacillus subtilis was constructed that allowed controlled switching between rod-shaped wild-type cells and corresponding L-forms. Both states can be stably maintained under suitable culture conditions. Because of the absence of a cell wall, L-forms are known to be insensitive to beta-lactam antibiotics, but reports on the susceptibility of L-forms to other antibiotics that interfere with membrane-anchored steps of cell wall biosynthesis are sparse, conflicting, and strongly influenced by strain background and method of L-form generation. Here we investigated the response of B. subtilis to the presence of cell envelope antibiotics, with regard to both antibiotic resistance and the induction of the known LiaRS- and BceRS-dependent cell envelope stress biosensors. Our results show that B. subtilis L-forms are resistant to antibiotics that interfere with the bactoprenol cycle, such as bacitracin, vancomycin, and mersacidin, but are hypersensitive to nisin and daptomycin, which both affect membrane integrity. Moreover, we established a lacZ-based reporter gene assay for L-forms and provide evidence that LiaRS senses its inducers indirectly (damage sensing), while the Bce module detects its inducers directly (drug sensing).
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