期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 41, 页码 14783-14788出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0505062102
关键词
cryo-EM; subviral particle; virus structure
资金
- NCRR NIH HHS [P41-RR01777, P41 RR001777] Funding Source: Medline
The infectious component of hepatitis B (HB) virus (HBV), the Dane particle, has a diameter of approximate to 44 nm and consists of a double-layered capsid particle enclosing a circular, incomplete double-stranded DNA genome. The outer capsid layer is formed from the HB surface antigen (HBsAg) and lipid, whereas the inner layer is formed from the HB core Ag assembled into an icosahedral structure. During chronic infection HBsAg is expressed in large excess as noninfectious quasispherical particles and tubules with approximate to 22-nm diameter. Here, we report cryo-EM reconstructions of spherical HBsAg particles at approximate to 12-angstrom resolution. We show that the particles possess different diameters and have separated them into two predominant populations, both of which have octahedral symmetry. Despite their differing diameters, the two forms of the particle have the same mass and are built through conformational switching of the same building block, a dimer of HBsAg. We propose that this conformational switching, combined with interactions with the underlying core, leads to the formation of HBV Dane particles of different sizes, dictated by the symmetry of the icosahedral core.
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