Indirect chemiluminescence detection for capillary zone electrophoresis of monoamines and catechol using luminol-K3[Fe(CN)6] system

Indirect chemiluminescence (ICL) detection for capillary electrophoresis (CE) of monoamines and catechol using luminol-K-3 [Fe(CN)(6)] system was described. A strong and stable background chemiluminescence (CL) signal can be generated by luminol-K-3[Fe(CN)(6)] reaction. Based on the principle of that some phenolic compounds may be oxidized in the presence of K-3[Fe(CN)(6)], quenching effect of catecholamines for luminol-K-3[Fe(CN)(6)] CL reaction results in a quantifiable decrease in the background signal. The conditions for CE separation and the CL detection for four standard catecholamines were systematically investigated using a homemade CE-ICL system. Under the optimum conditions, the detection limits of dopamine (DA), epinephrine (EP), norepinephrine (NE) and catechol (CA) were determined to be 0.18 mu M 0.39 mu M 0.48 mu M and 0.09 mu M, respectively. It also has been successfully applied to analyze seven pharmaceutical samples and seven human urine samples. (c) 2005 Elsevier B.V. All rights reserved.