期刊
FEMS MICROBIOLOGY LETTERS
卷 251, 期 2, 页码 243-249出版社
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2005.08.006
关键词
uvrA; ssb; ArcA/ArcB; two-component system; Escherichia coli
类别
The ArcA/ArcB two-component signal transduction system of Escherichia coli regulates gene expression in response to the redox conditions of growth. In this study, uvrA gene expression was repressed when ArcA was induced in E coli. Transcription of uvrA increased in Delta arc Delta and Delta arcB strains more than in the wild-type strain, whose trend was remarkable under the anaerobic condition. In the wild-type strain grown in the presence of DTT (10 mM), the uvrA gene expression was also repressed. Furthermore, the results of in vitro transcription and DNase I footprinting experiments indicated that ArcA specifically bound to the ArcA box [(A/T)GTTAATTA(A/T)] in the uvrA promoter and represses its transcription. These results suggest that the ArcA/ArcB two-component system works to negatively regulate uvrA gene expression. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据