期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 42, 页码 15012-15017出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0507596102
关键词
NuRD/MeCP1; HS2
资金
- NHLBI NIH HHS [IP01HL63357-06, N01-HV-28186, N01HV28186, P01 HL063357] Funding Source: Medline
- NIGMS NIH HHS [R37 GM048405, GM048405, R01 GM048405] Funding Source: Medline
Locus control regions (LCRs) are regulatory DNA sequences that are situated many kilobases away from their cognate promoters. LCRs protect transgenes from position effect variegation and heterochromatinization and also promote copy-number dependence of the levels of transgene expression. In this work, we describe the biochemical purification of a previously undescribed LCR-associated remodeling complex (LARC) that consists of heterogeneous nuclear ribonucleoprotein C1/C2, nucleosome remodeling SWI/ SNF, and nucleosome remodeling and deacetylating (NuRD)/ MeCP1 as a single homogeneous complex. LARC binds to the hypersensitive 2 (HS2)-Maf recognition element (MARE) DNA in a sequence-specific manner and remodels nucleosomes. Heterogeneous nuclear ribonucleoprotein C1/C2, previously known as a general RNA binding protein, provides a sequence-specific DNA recognition element for LARC, and the LARC DNA-recognition sequence is essential for the enhancement of transcription by HS2. Independently of the initiation of transcription, LARC becomes associated with beta-like globin promoters.
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