期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 336, 期 2, 页码 483-489出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2005.08.128
关键词
collagen; extracellular matrix; tension; degradation
The purpose of this investigation is to support the novel hypothesis that collagenous matrices are intrinsically smart load-adapting biomaterials. This hypothesis is based fundamentally on the postulate that tensile strain directly modulates the susceptibility of collagen molecules to enzymatic degradation (i.e., protects molecules which are under load from cleavage). To test this postulate, collagenase (Clostridiopeptidase A) was applied to a uniaxially loaded, anisotropic, devitalized, collagenous matrix in which a subset of fibrils was loaded in tension while the remaining fibrils carried little or no load. The collagen degradation pattern (as assessed by polarization and transmission electron microscopy) was found to correspond inversely to the tensile stress field such that fibrils under lower tensile load were preferentially cleaved. These results have immediate implications for tissue engineering of load-bearing collagenous matrices in vitro and may contribute significantly to our understanding of synthesis, remodelling, and pathogenesis of collagen matrices in vivo. (c) 2005 Elsevier Inc. All rights reserved.
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