4.5 Article

Identification of plasma membrane associated mature β-hexosaminidase A, active towards GM2 ganglioside, in human fibroblasts

期刊

FEBS LETTERS
卷 579, 期 25, 页码 5501-5506

出版社

WILEY
DOI: 10.1016/j.febslet.2005.08.081

关键词

beta-hexosaminidase; lysosomal enzyme; plasma membrane

资金

  1. NINDS NIH HHS [NS 09626] Funding Source: Medline

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Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of P-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, and by immunocytochemistry. The immunological and biochemical characterization of biotinylated beta-hexosaminidase A revealed that the plasma membrane associated enzyme is fully processed, suggesting its lysosomal origin. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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