期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1092, 期 2, 页码 246-249出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.08.025
关键词
hydrophilic interaction liquid chromatography (HILIC); monosaccharides; evaporative light scattering detection (ELSD)
Hydrophilic interaction liquid chromatography (HILIC) was used to separate monosaccharides that are common in N-linked oligosaccharides in glycoproteins and other compounds. A TSKgel Amide-80 column was eluted with 82% acetonitrile, in 5 mM ammonium formate (pH 5.5). Column temperature was 60 degrees C and evaporative light scattering was used for detection (ELSD). With this method, L-fucose, D-galactose, D-mannose, N-acetyl-D-glucosan-line, N-acetylneuraminic acid, and D-glucuronic acid were separated, with detection limits of 0.3-0.5 mu g for each monosaccharide, and intermediate precisions were 3-6% RSD (n = 6). (c) 2005 Elsevier B.V. All rights reserved.
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