期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 54, 期 11, 页码 4920-4923出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00349-10
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资金
- EU (European Resistance Fungal Network)
Aspergillus fumigatus sterol 14 alpha-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts were able to synthesize ergosterol and grow; a control strain harboring reverse-oriented cyp51A could not. YUG37-pcyp51A and YUG37-pcyp51B constructs showed identical sensitivity to itraconazole, posaconazole, clotrimazole, and voriconazole. Conversely, YUG37-pcyp51A withstood 16-fold-higher concentrations of fluconazole than YUG37-pcyp51B (8 and 0.5 mu g ml(-1), respectively).
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