期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 100, 期 7, 页码 789-793出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762005000700020
关键词
aminoglycoside resistance; polyamines; oligopeptide binding protein A; Escherichia coli; oligopeptide permease
The role of intracellular free polyamine (putrescine and spermidine) pools in multiple resistance to aminoglycoside antibiotics was investigated among in vitro selected kanamycin-resistant Escherichia coli J53 mutants expressing diminished oligopeptide-binding protein (OppA) levels and/or defective ornithine decarboxylase (ODC) activity. The results suggest that diminished OppA content, but not defective ODC activity expression, increased the relative concentration of free spermidine as compared to the wild type strain. Moreover by adding exogenous polyamines or polyamine synthesis inhibitors to cultures with different mutant strains, a direct relationship between the intracellular OppA levels and resistance to kanamycin was revealed. Collectively these results further suggest a complex relation among OppA expression, aminoglycoside resistance and polyamine metabolism.
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