期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 71, 期 11, 页码 7542-7547出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.71.11.7542-7547.2005
关键词
-
资金
- NIAID NIH HHS [R01 AI019844, R21 AI065352, R01 AI056177, R01 AI056177-03, R01 AI19844] Funding Source: Medline
In developing Clostridium perfringens as a safe vaccine vector, the alpha toxin gene (plc) in the bacterial chromosome must be permanently inactivated. Disrupting genes in C perfringens by traditional mutagenesis methods is very difficult. Therefore, we developed a new strategy using group 11 intron-based Target-Tron technology to inactivate the plc gene in C. perfringens ATCC 3624. Western blot analysis showed no production of alpha toxin protein in the culture supernatant of the plc mutant. Advantages of this technology, such as site specificity, relatively high frequency of insertion, and introduction of no antibiotic resistance genes into the chromosome, could facilitate construction of other C. perfringens mutants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据