Biosensing capability of gold-nanoparticle-immobilized three-dimensionally ordered macroporous film

A new type of biosensor was fabricated by coating gold nanoparticles onto a film with a three-dimensionally ordered macroporous (3DOM) structure. It was found that the localized surface plasmon resonance of the immobilized nanoparticles changes with the infiltration medium inside the voids of the 3DOM film and the surface-adsorbed compounds. Application of this type of film to the real-time detection of immunoreaction was demonstrated. It is anticipated that this kind of film will have applications in label-free biosensors.