期刊
EXPERIMENTAL CELL RESEARCH
卷 310, 期 2, 页码 383-391出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2005.08.015
关键词
bone marrow; mesenchymal; neural stem cells; juxtacrine; paracrine; transformation; plasticity; de-determination; differentiation; neurons
Several recent reports suggest that there is far more plasticity that previously believed in the developmental potential of bone-marrow-derived cells (BMCs) that can be induced by extracellular developmental signals of other lineages whose nature is still largely unknown. In this study, we demonstrate that bone-marrow-derived mesenchymal stem cells (MSCs) co-cultured with mouse proliferating or fixed (by paraformaldehyde or methanol) neural stem cells (NSCs) generate neural stem cell-like cells with a higher expression of Sox-2 and nestin when grown in NS-A medium supplemented with N2, NSC conditioned medium (NSCcm) and bFGF. These neurally induced MSCs eventually differentiate into beta-III-tubulin and GFAP expressing cells with neuronal and glial morphology when grown an additional week in Neurobasal/B27 without bFGF. We conclude that juxtacrine interaction between NSCs and MSCs combined with soluble factors released from NSCs are important for generation of neural-like cells from bone-marrow-derived adherent MSCs. (c) 2005 Elsevier Inc. All rights reserved.
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