期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 52, 期 12, 页码 4331-4337出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00506-08
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资金
- Italian Institute of Health [50G.30]
- Ministry of Instruction, University and Research [FIRB RBIP067F9E, FIRB RBPR05NWWC_006]
- Progetto Ateneo
The in vitro and in vivo activities of a killer decapeptide (KP) against influenza A virus is described, and the mechanisms of action are suggested. KP represents the functional internal image of a yeast killer toxin that proved to exert antimicrobial and anti-human immunodeficiency virus type 1 (HIV-1) activities. Treatment with KP demonstrated a significant inhibitory activity on the replication of two strains of influenza A virus in different cell lines, as evaluated by hemagglutination, hemadsorption, and plaque assays. The complete inhibition of virus particle production and a marked reduction of the synthesis of viral proteins (membrane protein and hemagglutinin, in particular) were observed at a KP concentration of 4 mu g/ml. Moreover, KP administered intraperitoneally at a dose of 100 mu g/mice once a day for 10 days to influenza A/NWS/33 (H1N1) virus-infected mice improved the survival of the animals by 40% and significantly decreased the viral titers in their lungs. Overall, KP appears to be the first anti-idiotypic antibody-derived peptide that displays inhibitory activity and that has a potential therapeutic effect against pathogenic microorganisms, HIV-1, and influenza A virus by different mechanisms of action.
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