期刊
JOURNAL OF FLUORESCENCE
卷 15, 期 6, 页码 883-894出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-005-0013-4
关键词
time-resolved emission spectroscopy; solvent relaxation; membrane curvature; antibacterial peptides; phase transition; dye relocalization
Although there exist a number of methods, such as NMR, X-ray, e.g., which explore the hydration of phospholipid bilayers, the solvent relaxation (SR) method has the advantage of simple instrumentation, easy data treatment and possibility of measuring fully hydrated samples. The main information gained from SR by the analysis of recorded time-resolved emission spectra (TRES) is microviscosity and micro-polarity of the dye microenvironment. Based on these parameters, one can draw conclusions about water structure in the bilayer. In this review, we focus on physical background of this method, on all the procedures that are needed in order to obtain relevant parameters, and on the requirements on the fluorescence dyes. Furthermore. a few recent applications (the effect of curvature, binding of antibacterial peptides and phase transition) illustrating the versatility of this method are mentioned. Moreover, limitations and potential problems are discussed.
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