期刊
PLANT AND CELL PHYSIOLOGY
卷 46, 期 11, 页码 1879-1883出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pci192
关键词
chalcone synthase; cucumber mosaic virus; Petunia hybrida; post-transcriptional gene silencing; RNA silencing; short interfering RNA
Petunia hybrida 'Red Star' is a variety whose flowers exhibit a star-type red and white bicolor pattern. We analyzed the mRNA levels of six genes involved in anthocyanin biosynthesis. Only the level of chalcone synthase (CHS) mRNA was depressed in the unpigmented flower sectors. Both transcriptional activity and the accumulation of short interfering RNA of CHS in the unpigmented sectors were detected. Viral infection blocked the generation of CHS-silenced sectors. These results indicate that sequence-specific degradation of CHS RNA is the primary cause of the formation of white sectors in 'Red Star' flowers.
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