期刊
BIOTECHNOLOGY LETTERS
卷 27, 期 21, 页码 1725-1730出版社
SPRINGER
DOI: 10.1007/s10529-005-2738-1
关键词
cell culture; flow cytometry; paclitaxel; protoplast; Taxol; Taxus
资金
- NIGMS NIH HHS [T32 GM08515] Funding Source: Medline
Single cells isolated from aggregated Taxus cuspidata cultures via enzymatic digestion were grown in suspension culture. High seeding density (similar to 4x10(5) cells/ml) and the addition of cell-free conditioned medium were essential for growth. Doubling the concentration of the nutrients [ascorbic acid (150 g/l), glutamine (6.25 mm), and citric acid (150 g/l)] had no effect on single cell growth or viability. A specific growth rate of 0.11 days(-1) was achieved, which is similar to the observed growth rate of aggregated Taxus suspensions. The biocide, Plant Preservative Mixture, added at 0.2% (v/v) to all single cell cultures to prevent microbial contamination, had no significant effect on growth or viability. Following cell sorting, single cell cultures can be used to establish new cell lines for biotechnology applications or provide cells for further study.
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