期刊
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
卷 81, 期 11, 页码 801-812出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09553000500531886
关键词
low dose radiation; nitric oxide; phagocytosis; CTL; CD69; IFN-gamma; CD8(+) T cells; immune response
Purpose: The aim of the present investigation was to study the effect of fractionated whole body low dose ionizing radiation (LDR) on the functional responses of T lymphocytes, their subpopulations and macrophages. Materials and methods: C57BL/6 mice were exposed to 4 cGy from a Co-60 source, at 0.31 cGy/min, at 24 h intervals for 5 days (total dose 20 cGy). Phagocytic activity was measured by flow cytometry using Bioparticles (R) and nitric oxide generation was estimated by spectrophotometry. Proliferation of lymphocytes in response to concanavalin A (con A) and alloantigens was measured by 3 H thymidine incorporation. Expression of cell surface markers was assessed by flow cytometric analysis of antibody labeled cells. Target cell killing by cytotoxic T cells (CTL) generated against allogenic cells was assessed by flow cytometry using PKH26 labeled target cells. Cytokines were estimated by enzyme linked immunosorbent assay. Results: Exposure to LDR enhanced nitric oxide secretion and phagocytosis. The expression of early activation antigen, CD69, was enhanced in CD8(+) T lymphocytes concomitant with enhanced proliferation in response to con A. In addition, mixed lymphocyte reaction (MLR) and CTL response were augmented and secretion of interferon gamma (IFN-gamma) was suppressed following LDR exposure. Conclusions: LDR exposure enhanced the function of macrophages and responses of CD8(+) T cells in C57BL/6 mice.
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