期刊
STEM CELLS
卷 23, 期 10, 页码 1541-1548出版社
WILEY
DOI: 10.1634/stemcells.2004-0338
关键词
human embryonic stem cells; sphingosine-1-phosphate; platelet-derived growth factor; lysophosphatidic acid
资金
- NIGMS NIH HHS [GM68417] Funding Source: Medline
Human embryonic stem cells (hESCs) have great potential for use in research and regenerative medicine, but very little is known about the factors that maintain these cells in the pluripotent state. We investigated the role of three major mitogenic agents present in serum-sphingosine-l-phosphate (SIP), lysophosphatidic acid (LPA), and platelet-derived growth factor (PDGF)-in maintaining hESCs. We show here that although LPA does not affect hESC growth or differentiation, coincubation of SIP and PDGF in a serum-free culture medium successfully maintains hESCs in an undifferentiated state. Our studies indicate that signaling pathways activated by tyrosine kinase receptors act synergistically with those downstream from lysophospholipid receptors to maintain hESCs in the undifferentiated state. This study is the first demonstration of a role for lysophospholipid receptor signaling in the maintenance of stem cell pluripotentiality. STEM C ELLS 2005;23:1541-1548.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据