期刊
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
卷 18, 期 13, 页码 1875-1884出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1871520618666180806104902
关键词
Acacia nilotica; pyrogallol; Helicobacter pylori; colon cancer; apoptosis; HT-29
资金
- Department of Science and Technology, New Delhi under DSTWOS-A scheme [DST/SR/WOS-A/LS-629/2012(G)]
Background: Colon cancer is the most aggressive form of cancers, that causes 0.5 million deaths per year around the globe. Targeting colon cancer by conventional therapeutic options elicits toxicity. Traditional medicines take a lead to alleviate the existing clinical challenges. Objective: To investigate antibacterial activity against Helicobacter Pylori and in vitro anti-colon cancer activity by Acacia nilotica extract (ACE) and its active constituent pyrogallol. Methods: Pyrogallol isolated from A. nilotica by column chromatography and HPLC and structure was elucidated by spectral analysis. Antibacterial activity was done by flow cytometry. Cytotoxicity was measured by MTT assay. Apoptotic morphology and nuclear fragmentation were assessed with AO/ethidium bromide and DAPI staining. DNA fragmentation was done by electrophoresis. Western blot used to analyze the molecular mechanism of apoptosis. Cell cycle arrest was determined using flow cytometry of propidium iodide stained cells. Cell migration was determined by wound healing assay. Results: ACE (20 mu g/ml) and pyrogallol (10 mu g/ml) treatment reduced the survival of H.pylori at 61% and 62%, respectively. MU results show that HT-29 cells are more sensitive to pyrogallol with an IC50 value of 35 mu g/ml compared to ACE. Pyrogallol treated HT-29 cells reached dead state i.e. late apoptotic state with severe nuclear fragmentation. Pyrogallol elicits dose dependent DNA fragmentation in HT-29 cells. Pyrogallol induced apoptosis by simultaneous down-regulation of Bcl-2 and up-regulation of BAX and cytochrome c. Pyrogallol arrested HT-29 cells in S and G2/M phase of cell cycle. Further pyrogallol exhibited marked anti-metastatic potential by inhibiting the migration of HT-29 cells dose dependently. Conclusion: Both ACE and pyrogallol repressed the growth of H.pylori and as significant anti-colon cancer agent.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据