Signal amplification in molecular imaging by pretargeting a multivalent, bispecific antibody

Here we describe molecular imaging of cancer using signal amplification of a radiotracer in situ by pretargeting a multivalent, bispecific antibody to carcinoembryonic antigen ( CEA), which subsequently also captures a radioactive hapten-peptide. Human colon cancer xenografts as small as similar to 0.15 g were disclosed in nude mice within 1 h of giving the radiotracer, with tumor/blood ratios increased by >= 40-fold ( similar to 10:1 at 1 h, similar to 100:1 at 24 h), compared to a Tc-99m-labeled CEA-specific F( ab') used clinically for colorectal cancer detection, while also increasing tumor uptake tenfold ( similar to 20% injected dose/g) under optimal conditions. This technology could be adapted to other antibodies and imaging modalities.