期刊
JOURNAL OF VIROLOGY
卷 79, 期 22, 页码 14179-14188出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.79.22.14179-14188.2005
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资金
- NHLBI NIH HHS [K08 HL03984, T32 HL007775] Funding Source: Medline
- NIAID NIH HHS [R01 AI058862, R01 AI058862-01] Funding Source: Medline
- PHS HHS [K08 A50458-01] Funding Source: Medline
Resting CD4(+) T cells are the best-defined reservoir of latent human immunodeficiency virus type 1 (HIV-1) infection, but how the reservoir is formed is unclear. Understanding how the reservoir of latently infected cells forms is critical because it is a major barrier to curing HIV infection. The system described here may provide an in vitro model of latent HIV-1 infection in resting CD4(+) T cells. We demonstrated that HIV-1 integrates into the genomes of in vitro-inoculated resting CD4(+) T cells that have not received activating stimuli and have not entered cell cycle stage G(1b). A percentage of the resting CD4(+) T cells that contain integrated DNA produce virus upon stimulation, i.e., are latently infected. Our results show that latent HIV-1 infection occurs in unstimulated resting CD4(+) T cells and suggest a new route for HIV-1 reservoir formation.
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