期刊
JOURNAL OF NEUROSCIENCE
卷 25, 期 44, 页码 10247-10251出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1089-05.2005
关键词
action potential; low-threshold calcium channel; calcium channels; dihydropyridine; L-type; alpha 1D; alpha 1C; Ca(V)1.2; Ca(V)1.3
资金
- NIMH NIH HHS [MH19118, T32 MH019118] Funding Source: Medline
- NINDS NIH HHS [NS29967, R01 NS029967] Funding Source: Medline
Neuronal L-type calcium channels are essential for regulating activity-dependent gene expression, but they are thought to open too slowly to contribute to action potential-dependent calcium entry. A complication of studying native L-type channels is that they represent a minor fraction of the whole-cell calcium current in most neurons. Dihydropyridine antagonists are therefore widely used to establish the contribution of L-type channels to various neuronal processes and to study their underlying biophysical properties. The effectiveness of these antagonists on L-type channels, however, varies with stimulus and channel subtype. Here, we study recombinant neuronal L-type calcium channels, Ca(v)1.2 and Ca(v)1.3. We show that these channels open with fast kinetics and carry substantial calcium entry in response to individual action potential waveforms, contrary to most studies of native L-type currents. Neuronal Ca(v)1.3 L-type channels were as efficient as Ca(v)2.2 N-type channels at supporting calcium entry during action potential-like stimuli. We conclude that the apparent slow activation of native L-type currents and their lack of contribution to single action potentials reflect the state-dependent nature of the dihydropyridine antagonists used to study them, not the underlying properties of L-type channels.
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