期刊
NEUROSCIENCE LETTERS
卷 388, 期 1, 页码 49-53出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2005.06.030
关键词
amino acids; transaminases; human CSF; human brain; Alzheimer disease
The present study was conducted to determine the concentration of amino acids in the cerebrospinal spinal fluid (CSF) and the activities of two tramsaminases: glutamic oxaloacetate transaminase (GOT) and glutamic pyruvate transaminase (GPT) in human Alzheimer disease (AD) and normal brain. L-Glutamic acid, L-glutamine and L-alanine are the most abundant amino acids in the CSF (50-55% of total amino acids). L-Glutamine occurs at much higher levels in Alzheimer CSF compared to the normal CSF (229 +/- 91.8 nmol/ml in AD versus 107 +/- 47.2 nmol/ml in normal; P=0.0041). In contrast, L-aspartate occurs at significantly lower concentrations in Alzheimer CSF than normal CSF (46.1 +/- 25.7 nmol/ml in Alzheimer versus 95.2 +/- 52.6 nmol/ml in normal; P=0.020). In Alzheimer brain (frontal, parietal and occipital cortices) GOT is present at significantly higher activities than in normal brain cortices (about 1.5 times higher; P<0.01). No significant differences for GPT activity occurred between normal and AD brain. Since CSF receives amino acids from brain tissues, and since GOT catalyzes the conversion of L-aspartate to L-glutamate, the higher concentrations Of L-glutamine (which is derived from L-glutamate), and the lower concentrations of L-aspartate found in Alzheimer CSF could be considered as a consequence of the higher activity of GOT that occurs in Alzheimer brain. (C) 2005 Elsevier Ireland Ltd. All rights reserved.
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