期刊
NEUROREPORT
卷 16, 期 16, 页码 1735-1739出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/01.wnr.0000185958.03841.0f
关键词
dorsal root ganglion; heat transduction; pain; transient receptor potential; vanilloid receptors
Coassociation of the vanilloid transient receptor potential (Trp) ion channels, TrpV1 and TrpV2, was investigated by immunoprecipitation and immunofluorescence in transfected mammalian cell lines, rat dorsal root ganglia and spinal cord. TrpV1/TrpV2 heteromeric complexes were coimmunoprecipitated from human embryonic kidney cells and F-II dorsal root ganglion hybridoma cells following their transient coexpression. Immunofluorescent labelling of transfected F-II cells revealed colocalization of TrpVl and TrpV2 at the cell surface. Immunoprecipitation from rat dorsal root ganglion lysates identified a minor population of receptor complexes composed of TrpV1/TrpV2 heteromers, consistent with a small proportion of cells double-labelled with TrpVl and TrpV2 antibodies in rat dorsal root ganglion sections. TrpV1/TrpV2 receptor complexes may represent a functionally distinct ion channel complex that may increase the diversity observed within the Trp ion channel family.
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