期刊
FEBS LETTERS
卷 579, 期 27, 页码 6111-6114出版社
WILEY
DOI: 10.1016/j.febslet.2005.09.081
关键词
beta-carotene ketolase; carotenoid; crtO; crtW; cyanobacteria; Anabaena sp PCC 7120
Two beta-carotene ketolases, CrtW and CrtO, are widely distributed in bacteria, although they show no significant sequence homology with each other. The cyanobacterium Anabaena sp. PCC 7120 was found to have two homologous genes. In the crtW deleted mutant, myxol 2 '-fucoside was present, but ketomyxol 2 '-fucoside was absent. In the crtO deleted mutant, P-carotene was accumulated, and the amount of echinenone was decreased. Therefore, CrtW catalyzed myxol 2 '-fucoside to ketomyxol 2 '-fucoside, and CrtO catalyzed beta-carotene to echinenone. This cyanobacterium was the first species found to have both enzymes, which functioned in two distinct biosynthetic pathways. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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