期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 45, 页码 16368-16373出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0507904102
关键词
colorectal cancer; plasma DNA; tumor suppressor gene; circulating DNA; diagnosis
资金
- NCI NIH HHS [CA62924, CA43460, R37 CA057345, R01 CA057345, CA57345, R37 CA043460, P50 CA062924] Funding Source: Medline
The early detection of cancers through analysis of circulating DNA could have a substantial impact on morbidity and mortality. To achieve this goal, it is essential to determine the number of mutant molecules present in the circulation of cancer patients and to develop methods that are sufficiently sensitive to detect these mutations. Using a modified version of a recently developed assay for this purpose, we found that patients with advanced colorectal cancers consistently contained mutant adenomatous polyposis coli (APC) DNA molecules in their plasma. The median number of APC DNA fragments in such patients was 47,800 per ml of plasma, of which 8% were mutant. Mutant APC molecules were also detected in > 60% of patients :with early, presumably curable colorectal cancers, at levels ranging from 0.01% to 1.7% of the total APC molecules. These results have implications for the mechanisms through which tumor DNA is released into the circulation and for diagnostic tests based on this phenomenon.
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