期刊
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 65
卷 65, 期 -, 页码 175-196出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-physchem-040513-103644
关键词
biomolecule; conformation; solution structure; gas-phase structure; IMS-IMS
资金
- NIGMS NIH HHS [RC1GM090797-02] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [RC1GM090797] Funding Source: NIH RePORTER
The combination of mass spectrometry and ion mobility spectrometry (IMS) employing a temperature-variable drift cell or a drift tube divided into sections to make IMS-IMS experiments possible allows information to be obtained about the molecular dynamics of polyatomic ions in the absence of a solvent. The experiments allow the investigation of structural changes of both activated and native ion populations on a timescale of 1-100 ms. Five different systems representing small and large, polar and nonpolarmolecules, as well as noncovalent assemblies, are discussed in detail: a dinucleotide, a sodiated polyethylene glycol chain, the peptide bradykinin, the protein ubiquitin, and two types of peptide oligomers. Barriers to conformational inter-conversion can be obtained in favorable cases. In other cases, solution-like native structures can be observed, but caremust be taken in the experimental protocols. The power of theoretical modeling is demonstrated.
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