期刊
FEMS MICROBIOLOGY LETTERS
卷 252, 期 2, 页码 223-228出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.femsle.2005.08.049
关键词
Amanita exitialis; amatoxins; HPLC; ITS; MS
类别
Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the pure culture and of a typical fruiting body of the species were sequenced and compared. Further, amatoxins produced by pure cultures were analyzed and characterized by high-performance liquid chromatography and mass spectrometry analysis. The results showed that the pure cultures produced 728.3 +/- 43.8 mu g g(-1) (dry matter) of a-Amanitin and 60.0 +/- 20.7 mu g g(-1) (dry matter) of beta-Amanitin, respectively, a yield which is about 10% of that produced by fruiting bodies. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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