期刊
ANNUAL REVIEW OF MICROBIOLOGY, VOL 67
卷 67, 期 -, 页码 179-197出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-micro-092412-155637
关键词
channel; secretion; toxin; porin; patch clamp; planar bilayer
类别
资金
- NIAID NIH HHS [AI068943, AI34905] Funding Source: Medline
- NIGMS NIH HHS [GM062987] Funding Source: Medline
Bacteria secrete and harbor in their membranes a number of pore-forming proteins. Some of these are bona fide ion channels that may respond to changes in membrane tension, voltage, or pH. Others may be large translocons used for the secretion of folded or unfolded polypeptide substrates. Additionally, many secreted toxins insert into target cell membranes and form pores that either collapse membrane electrochemical gradients or provide conduits for the delivery of virulence factors. In all cases, electrophysiological approaches have yielded much progress in past decades in understanding the functional mechanisms of these pores. By monitoring the changes in current due to ion flow through the pores, these techniques are used as high-resolution tools to gather detailed information on the kinetic and permeation properties of these proteins, including those whose physiological role is not ion flux. This review highlights some of the electrophysiological studies that have advanced the field of transport by pore-forming proteins of bacterial origin.
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