期刊
JOURNAL OF COMPARATIVE NEUROLOGY
卷 492, 期 3, 页码 323-333出版社
WILEY
DOI: 10.1002/cne.20739
关键词
efferent; hair cells; crista ampullaris; otolith; macular organ; vestibular ganglion
资金
- NIDCD NIH HHS [R01 DC003086, R01 DC02521, R01 DC002521-10, R01 DC03086, R01 DC002521] Funding Source: Medline
Vestibular tissues (cristae ampullares, macular otolithic organs, and Scarpa's ganglia) in chinchilla, rat, and guinea pig were examined for immunoreactivity to the alpha 9 nicotinic acetylcholine receptor (nAChR) subunit. The a9 antibody was generated against a conserved peptide present in the intracellular loop of the predicted protein sequence of the guinea pig alpha 9 nAChR subunit. In the vestibular periphery, staining was observed in calyces around type I hair cells, at the synaptic pole of type II hair cells, and in varying levels in Scarpa's ganglion cells. Ganglion cells were also triply labeled to detect alpha 9, calretinin, and peripherin. Calretinin labels calyx-only afferents. Peripherin labels bouton-only afferents. Dimorphic afferents, which have both calyx and bouton endings, are not labeled by calretinin or peripherin. In these experiments, alpha 9 was expressed in both calyx and dimorphic afferents. A subpopulation of small ganglion cells did not contain the alpha 9 nAChR but did stain for peripherin. We surmise that these are bouton-only afferents. Bouton (regularly discharging) afferents also show efferent responses, although they are qualitatively different from those in irregularly discharging (calyx and dimorphic) afferents, much slower and longer lasting. Thus, regular afferents are probably more affected via a muscarinic cholinergic or a peptidergic mechanism, with a much smaller superimposed fast nicotinic-type response. This latter response could be due to one of the other nicotinic receptors that have been described in studies from other laboratories.
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