期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 337, 期 3, 页码 892-900出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2005.09.128
关键词
amino acid transport; cotransporters; neurotransmitter synthesis; sodium-dependent transport; Xenopus oocyte
The SLC6 gene family comprises membrane proteins that transport neurotransmitters, amino acids, or osmolytes. We report the first functional characterization of the human SLC6A15 gene, which codes for a sodium-coupled branched-chain amino-acid transporter I (SBAT1). SBAT1 expression is specific to the brain. When expressed in Xenopus oocytes, SBATI mediated Na+-coupled transport of hydrophobic, zwitterionic a-amino and imino acids. SBAT1 exhibited a strong preference for branched-chain amino acids (BCAA) and methionine (K-0.5 80-160 mu M). SBAT1 excluded aromatic or charged amino acids, P-amino acids, glycine, and GABA. SBAT1-mediated transport of amino or imino acids was extremely temperature-dependent (Q(10) = 9) and was inhibited at acidic pH. PKC activation reduced the plasma-membrane population of SBATI protein. SBAT1-mediated transport of BCAA, particularly leucine, may be an important source of amino nitrogen for neurotransmitter synthesis in glutamatergic and GABAergic neurons. (c) 2005 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据