期刊
ANNUAL REVIEW OF GENOMICS AND HUMAN GENETICS
卷 10, 期 -, 页码 135-151出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-genom-082908-145957
关键词
next-generation sequencing technology; Illumina/Solexa; 454/Roche; ABI SOLiD; Helicos HeliScope; RNA sequencing
资金
- National Cancer Institute of Canada, Genome Canada, Genome British Columbia
- British Columbia Cancer Foundation
- Michael Smith Foundation
- Natural Sciences and Engineering Research Council of Canada
Transcriptome analysis has been a key area of biological inquiry for decades. Over the years, research in the field has progressed from candidate gene-based detection of RNAs using Northern blotting to high-throughput expression profiling driven by the advent of microarrays. Next-generation sequencing technologies have revolutionized transcriptomics by providing opportunities for multidimensional examinations of cellular transcriptomes in which high-throughput expression data are obtained at a single-base resolution.
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