期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 78, 期 6, 页码 1291-1300出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0605342
关键词
T helper 1 cytokines; inflammatory bowel disease; intestinal inflammation
资金
- NCI NIH HHS [P30 CA43703] Funding Source: Medline
- NIDDK NIH HHS [DK-046461, DK-57756, DK-46461-11S1] Funding Source: Medline
Intestinal immune responses are normally regulated to maintain a state of immune balance. Dendritic cells (DC) are antigen-presenting cells, which induce immune responses against microbes and other stimuli and are key players in the regulation of tolerance in the gut. These cells influence the differentiation of cytokine responses in T cells, and in the gut, in particular, such interactions may be critical to the course of inflammatory bowel disease (IBD). Using the CD45RBhi CD4(+) T cell-reconstituted severe combined immunodeficient mouse model of colitis, we investigated the ability of isolated colon DC to stimulate immune responses in syngeneic and allogeneic spleen CD4+ T cells, as well as in colon T cells isolated from the same tissue as DC in IBC mice. We found that the frequency of DC in IBC mice colons and spleens was elevated in comparison with control mice, but colon and spleen DC exhibited different phenotypic and functional properties. Colon DC stimulated significantly higher levels of interferon-gamma and interleukin-6 when cocultured with autologous colon T cells than in cocultures with syngeneic or allogeneic spleen T cells. These data suggest that in the IBD colon, DC-T cell interactions may create conditions with an abundance of proinflammatory cytokines, which favor the inflammatory state.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据