Kinetics of pulmonary VO2 and femoral artery blood flow and their relationship during repeated bouts of heavy exercise

The mechanism that alters the pulmonary VO2 response to heavy-intensity exercise following prior heavy exercise has been frequently ascribed to an improvement in pre-exercise blood flow (BF) or O-2 delivery. Interventions to improve O-2 delivery have rarely resulted in a similar enhancement of VO2: However, the actual limb blood flow and VO2 dynamics in the second bout of repeated exercise remain equivocal. Seven healthy female subjects (21 - 32 years) performed consecutive 6-min (separated by 6 min of 10 W exercise) bilateral knee extension (KE) exercise in a semisupine position at a work rate halfway between the lactate threshold (LT) and VO2 peak. Femoral artery blood flow (FBF) was measured by Doppler ultrasound simultaneously with breath-by-breath VO2; each protocol being repeated at least four times for precise kinetic characterization. The effective time-constant (tau') of the VO2 response was reduced following prior exercise ( bout 1: 61.0 +/- 10.5 vs. bout 2: 51.6 +/- 9.0 s; mean +/- SD; P< 0.05), which was a result of a reduced slow component (bout 1: 16.0 +/- 8.0 vs. bout 2: 12.5 +/- 6.7 %; P< 0.05) and an unchanged 'primary' tau. FBF was consistently faster than VO2. However, there was no bout-effect on tau' FBF (bout 1: 28.2 +/- 12.0 vs. bout 2: 34.2 +/- 8.5 s). The relationship between the exercise-associated VO2 (i.e ., Delta VO2) and Delta FBF was similar between bouts, with a tendency (N.S: P>0.05) for Delta VO2/Delta FBF to be increased during the transition to bout 2 rather than decreased, as hypothesized. The return of VO2 kinetics toward first order, therefore, was associated with an 'appropriate', not enhanced, BF to the working muscles. Whether a relative prior-hyperemia in bout 2 enables a more homogeneous intramuscular distribution of BF and/or metabolic response is unclear, however, these data are consistent with events more proximal to the exercise muscle in mediating the _ VO2 response during repeated heavy-intensity KE exercise.