期刊
FUNGAL GENETICS AND BIOLOGY
卷 42, 期 12, 页码 989-998出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2005.09.003
关键词
Cryptococcus neoformans; melanin
资金
- NCI NIH HHS [T32CA09173] Funding Source: Medline
- NIAID NIH HHS [AI52733, AI060507] Funding Source: Medline
- NIGMS NIH HHS [GM64100] Funding Source: Medline
Cryptococcus neoformans produces pigments in vitro in the presence of exogenous substrate. We characterized acid-resistant particles isolated from pigmented cells grown in L-dopa, methyl-dopa, (-)-epinephrine or (-)-norepinephrine. The goals of this study were to determine whether pigments made from each of these substrates were melanins and the consequences of pigmentation on related cell characteristics. The greatest yield of acid-resistant particles occurred with methyl-dopa followed by L-dopa. Electron microscopy indicated that L-dopa and methyl-dopa produced particles with thicker shells. The mAb 6D2 reacted with all particles, but a lower reactivity was observed with epinephrine-derived particles. ESR analysis revealed that epinephrine-derived particles failed to produce a stable free radical signal typical of melanins. Growth of C neoformans in different substrates affected cell and capsule size but not capsule induction. Hence, the type of pigment produced by C neoformans is dependent on the substrate and not all pigments meet the criteria for melanins. (c) 2005 Elsevier Inc. All rights reserved.
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