期刊
ANNUAL REVIEW OF BIOPHYSICS, VOL 39
卷 39, 期 -, 页码 139-158出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev.biophys.050708.133630
关键词
free interface diffusion; high throughput; screening; phase diagram; in situ diffraction
类别
资金
- NIGMS NIH HHS [GM074961, 5 R01 GM075827] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM075827, U54GM074961] Funding Source: NIH RePORTER
To obtain protein crystals, researchers must search for conditions in multidimensional chemical space. Empirically, thousands of crystallization experiments are carried out to screen various precipitants at multiple concentrations. Microfluidics can manipulate fluids on a nanoliter scale, and it affects crystallization twofold. First, it miniaturizes the experiments that can currently be done on a larger scale and enables crystallization of proteins that are available only in small amounts. Second, it offers unique experimental approaches that are difficult or impossible to implement on a larger scale. Ongoing development of microfluidic techniques and their integration with protein production, characterization, and in situ diffraction promises to accelerate the progress of structural biology.
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