期刊
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 14
卷 14, 期 -, 页码 351-367出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-bioeng-071811-150108
关键词
fluorescence; microscopy; redox ratio; lifetime imaging; two-photon excited fluorescence; NADH; FAD
资金
- NIAMS NIH HHS [F32AR061933] Funding Source: Medline
- NIBIB NIH HHS [R01EB007542] Funding Source: Medline
Optical microscopic imaging offers opportunities to perform noninvasive assessments of numerous parameters associated with the biochemistry, morphology, and functional state of biological samples. For example, it is possible to detect the endogenous fluorescence from a small number of important biomolecules, including NADH and FAD, which are two coenzymes involved in key metabolic pathways such as glycolysis, the Krebs cycle, and oxidative phosphorylation. Here, we review different imaging approaches to isolate the fluorescence from these chromophores in two- and three-dimensional samples and discuss the origins and potential interpretation of the observed signals in terms of cell metabolic status. Finally, we discuss the challenges and limitations of these approaches, as well as important research directions that we expect will evolve in the near future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据