期刊
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 4
卷 4, 期 -, 页码 419-437出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev.anchem.111808.073722
关键词
second harmonic generation; protein crystallization; drug formulation
资金
- NATIONAL CENTER FOR RESEARCH RESOURCES [R01RR026273] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P50GM088499, R01GM103401] Funding Source: NIH RePORTER
- NCRR NIH HHS [1R01RR026273, R01 RR026273-01, R01 RR026273] Funding Source: Medline
- NIGMS NIH HHS [R01 GM103401, 1P50GM088499, P50 GM088499] Funding Source: Medline
Second-order nonlinear optical imaging of chiral crystals (SONICC) is an emerging technique for crystal imaging and characterization. We provide a brief overview of the origin of second harmonic generation signals in SONICC and discuss recent studies using SONICC for biological applications. Given that they provide near-complete suppression of any background, SONICC images can be used to determine the presence or absence of protein crystals through both manual inspection and automated analysis. Because SONICC creates high-resolution images, nucleation and growth kinetics can also be observed. SONICC can detect metastable, homochiral crystalline forms of amino acids crystallizing from racemic solutions, which confirms Ostwald's rule of stages for crystal growth. SONICC's selectivity, based on order, and sensitivity, based on background suppression, make it a promising technique for numerous fields concerned with chiral crystal formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据