期刊
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY
卷 2, 期 -, 页码 241-264出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev.anchem.1.031207.112851
关键词
optical; electrochemical; amplification; nanoparticle
资金
- Welch Foundation
- National Institutes of Health
- National Institute of Biomedical Imaging and Bioengineering
Aptamers are ligand-binding nucleic acids whose affinities and selectivities can rival those of antibodies. They have been adapted to analytical applications not only as alternatives to antibodies, but as unique reagents in their own right. In particular, aptamers can be readily site-specifically modified during chemical or enzymatic synthesis to incorporate particular reporters, linkers, or other moieties. Also, aptamer secondary structures can be engineered to undergo analyte-dependent conformational changes, which, in concert with the ability to specifically place chemical agents, opens Lip a wealth of possible signal transduction schemas, irrespective of whether the detection modality is optical, electrochemical, or mass based. Finally because, aptamers are nucleic acids, they ire readily adapted to sequence- (and hence signal-) amplification methods. However, application of aptamers without a basic knowledge of their biochemistry or technical requirements call cause serious analytical difficulties.
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