期刊
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY
卷 1, 期 -, 页码 165-190出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev.anchem.1.031207.113104
关键词
fluorescence; capillary electrophoresis; single-cell analysis; cytometry
资金
- National Institutes of Helath [R01NS061767-01, R01GM071666, R33CA122900, P50HG002360]
- NATIONAL CANCER INSTITUTE [R33CA122900] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [P50HG002360] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM071666] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS061767] Funding Source: NIH RePORTER
Cytometry deals with the analysis of the composition of single cells. Flow and image cytometry employ, antibody-based stains to characterize a handful of components in single cells. Chemical cytometry in contrast, employs a suite of powerful analytical tools to characterize a large number of components. Tools have been developed to characterize nucleic acids, proteins, and metabolites in single cells. Whereas nucleic acid analysis employs powerful polymerase chain reaction-based amplification techniques, protein and metabolite analysis tends to employ capillary electrophoresis separation and ultrasensitive laser-induced fluorescence detection. It is now possible to detect yoctomole amounts of many analytes in single cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据